Fig. 3From: Metallothionein-3 is a multifunctional driver that modulates the development of sorafenib-resistant phenotype in hepatocellular carcinoma cellsIn Huh7 cells, hMT3 affects mitochondrial flux, oxidative stress, apoptosis, and cell cycle. Metabolic analysis of Huh7mock and Huh7hMT3 cells expressed as (A) OCR and (B) ECAR values (n = 6). C Sorafenib-mediated induction of total ROS, (D) mitochondrial superoxide and (E) SOD activity in Huh7mock and Huh7hMT3 cells. *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0005 (Student’s test, two-sided). F Scatter plots of double-stained (Annexin V-Dy647/7-AAD) cells showing markedly lower ability of sorafenib to trigger apoptosis in Huh7hMT3 and BCLC-3WT compared to Huh7mock cells. G Quantification of flow cytometry analysis of apoptosis induction by sorafenib. Data are expressed as mean ± SEM. ***p ≤ 0.0005 (Student’s test, two-sided). H Cell cycle histograms of PI-stained cells cultured with or without sorafenib. I Bar graphs showing the quantification of the frequencies of cells in individual cell cycle phases. Data are expressed as mean ± SEM. **p ≤ 0.001 (Student’s test, two-sided)Back to article page