Fig. 4
From: HMGA1 augments palbociclib efficacy via PI3K/mTOR signaling in intrahepatic cholangiocarcinoma
Potential role of PI3K/mTOR inhibitors in iCCA treatment.
A, PF-04691502 drug sensitivity half maximal inhibitory concentration (IC50) values (nM).
B, Western blot analysis of related genes in iCCA cell lines treated with PF-04691502. Protein lysates were collected after drug treatment for 48Â h.
C-D, PF-04691502 inhibited the proliferation, migration and invasion of iCCA cell lines. Cell proliferation was evaluated with CCK − 8 assay. Cell migration and invasion were investigated with transwell assays.
E-F, Western blot analysis of EMT-related factors and cell stemness-related proteins in iCCA cell lines HUCCT1 and RBE cells treated with PF-04691502, or control. Protein lysates were collected after drug treatment for 48Â h.
G-H, Images showing 3D sphere formation and colony formation assays of iCCA cell lines, after 3000nM(HUCCT1) or 500 nM(RBE) PF-04691502 treatment for 9 days.
Data were shown as mean ± SEM. n.s. represents not significant. *p < 0.05, **p < 0.01 and ***p < 0.001 as calculated by the one-way or two-way ANOVA. Data from one representative experiment are presented (n = 3)